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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
07/07/2021 |
Actualizado : |
02/09/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
DONCEL, B.; CASTELLS, M.; MAYA, L.; FRAGA, M.; UZAL, F.A; COLINA, R.; GIANNITTI, F. |
Afiliación : |
BENJAMÍN DONCEL DÍAZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MATÍAS CASTELLS, Laboratorio de Virología Molecular, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República (UdelaR), Salto, Uruguay.; LETICIA MAYA, Laboratorio de Virología Molecular, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República (UdelaR), Salto, Uruguay.; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FRANCISCO A UZAL, California Animal Health and Food Safety (CAHFS) Laboratory, University of California at Davis, San Bernardino, California, Estados Unidos.; RODNEY COLINA, Laboratorio de Virología Molecular, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República (UdelaR), Salto, Uruguay.; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Encefalitis asociada a astrovirus bovino neurotrópico, ¿una enfermedad subdiagnosticada en Sudamérica?.[Neurotropic bovine astrovirus-associated encephalitis: An underdiagnosed disease in South America?]. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Revista Argentina de Microbiologia, Volume 54, Pages 100-105, 2022. OPEN ACCESS. Doi: https://doi.org/10.1016/j.ram.2021.01.00 |
DOI : |
10.1016/j.ram.2021.01.00 |
Idioma : |
Español |
Notas : |
Article history: Recibido el 15 de julio de 2020/ Aaceptado el 24 de enero de 2021./Disponible en Internet el 18 junio 2021. Autor para correspondencia: Correo electrónico: fgiannitti@inia.org.uy (F. Giannitti). Este trabajo fue financiado por el proyecto PL 27 N-23398 y la beca de posgrado 1070-2018 del INIA, Uruguay. |
Contenido : |
Resumen: Describimos un caso de encefalitis asociada a infección por astrovirus bovino neurotrópico en una vaca lechera, raza Jersey, del departamento de San José, Uruguay. Este representa el segundo caso reportado de esta condición en el hemisferio sur. La vaca, única
afectada de un rodeo de 70 bovinos, manifestó signos clínicos neurológicos con curso de 2 días, luego de los que murió espontáneamente. El examen histopatológico reveló meningoencefalitis linfocítica, histiocítica y plasmacítica, con necrosis neuronal, sin cuerpos de inclusión. No se
detectaron en el cerebro otros agentes infecciosos, incluyendo el virus de la rabia (Lyssavirus), alfaherpesvirus bovino-1 y alfaherpesvirus bovino-5 (Varicellovirus), virus de la diarrea viral bovina (Pestivirus), virus del Nilo Occidental (Flavivirus), Listeria monocytogenes, Histophilus somni y otras bacterias. Dado que el descubrimiento de astrovirus neurotrópicos en varias especies de mamíferos, incluidos humanos, es reciente, proponemos que los casos de encefalitis por astrovirus pudieron haber pasado inadvertidos en Sudamérica. Discutimos brevemente
el diagnóstico patológico diferencial de encefalitis infecciosas en bovinos. bstract We describe a case of neurotropic bovine astrovirus-associated encephalitis in a Jersey dairy cow from the department of San José, Uruguay. This represents the second case of this
condition reported in the Southern Hemisphere. The cow was the only one affected in a herd of 70 cows, showing neurological signs with a 2-day clinical course, before dying spontaneously. Histopathological examination revealed lymphocytic, histiocytic, and plasmacytic meningoencephalitis with neuronal necrosis, without detectable inclusion bodies. Other infectious agents, including Rabies virus (Lyssavirus), Bovine alphaherpesvirus-1 and Bovine alphaherpesvirus-5 (Varicellovirus), Bovine viral diarrhea virus (Pestivirus), West Nile virus (Flavivirus), Listeria monocytogenes, Histophilus somni and other bacteria, were not detected in the brain. We propose that given the recent discovery of neurotropic astroviruses in various mammalian species, including humans, cases of astrovirus encephalitis may have gone undetected in South America.We briefly discuss the differential pathologic diagnosis of infectious bovine encephalitis.
Abstract We describe a case of neurotropic bovine astrovirus-associated encephalitis in a Jersey dairy cow from the department of San José, Uruguay. This represents the second case of this condition reported in the Southern Hemisphere. The cow was the only one affected in a herd of 70 cows, showing neurological signs with a 2-day clinical course, before dying spontaneously. Histopathological examination revealed lymphocytic, histiocytic, and plasmacytic meningoencephalitis with neuronal necrosis, without detectable inclusion bodies. Other infectious agents,including Rabies virus (Lyssavirus), Bovine alphaherpesvirus-1 and Bovine alphaherpesvirus-5 (Varicellovirus), Bovine viral diarrhea virus (Pestivirus), West Nile virus (Flavivirus), Listeria
monocytogenes, Histophilus somni and other bacteria, were not detected in the brain. We propose that given the recent discovery of neurotropic astroviruses in various mammalian species,including humans, cases of astrovirus encephalitis may have gone undetected in South America.
We briefly discuss the differential pathologic diagnosis of infectious bovine encephalitis.
© 2021 Asociacion´ Argentina de Microbiolog´?a MenosResumen: Describimos un caso de encefalitis asociada a infección por astrovirus bovino neurotrópico en una vaca lechera, raza Jersey, del departamento de San José, Uruguay. Este representa el segundo caso reportado de esta condición en el hemisferio sur. La vaca, única
afectada de un rodeo de 70 bovinos, manifestó signos clínicos neurológicos con curso de 2 días, luego de los que murió espontáneamente. El examen histopatológico reveló meningoencefalitis linfocítica, histiocítica y plasmacítica, con necrosis neuronal, sin cuerpos de inclusión. No se
detectaron en el cerebro otros agentes infecciosos, incluyendo el virus de la rabia (Lyssavirus), alfaherpesvirus bovino-1 y alfaherpesvirus bovino-5 (Varicellovirus), virus de la diarrea viral bovina (Pestivirus), virus del Nilo Occidental (Flavivirus), Listeria monocytogenes, Histophilus somni y otras bacterias. Dado que el descubrimiento de astrovirus neurotrópicos en varias especies de mamíferos, incluidos humanos, es reciente, proponemos que los casos de encefalitis por astrovirus pudieron haber pasado inadvertidos en Sudamérica. Discutimos brevemente
el diagnóstico patológico diferencial de encefalitis infecciosas en bovinos. bstract We describe a case of neurotropic bovine astrovirus-associated encephalitis in a Jersey dairy cow from the department of San José, Uruguay. This represents the second case of this
condition reported in the Southern Hemisphere. The cow was the only one affected in a herd of 70 cows, showing neuro... Presentar Todo |
Palabras claves : |
Astrovirus bovino; BOVINE ASTROVIRUS; DAIRY COWS; Enfermedad neurológica; Neurological disease; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; PLATAFORMA DE SALUD ANIMAL. |
Thesagro : |
URUGUAY; VACAS LECHERAS. |
Asunto categoría : |
E16 Enfermedades de los animales |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16661/1/Revista-Argentina-de-Microbiologia-54-2022-100-105.pdf
https://www.sciencedirect.com/science/article/pii/S0325754121000547/pdfft?isDTMRedir=true&download=true
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Marc : |
LEADER 04975naa a2200325 a 4500 001 1062283 005 2022-09-02 008 2022 bl uuuu u00u1 u #d 024 7 $a10.1016/j.ram.2021.01.00$2DOI 100 1 $aDONCEL, B. 245 $aEncefalitis asociada a astrovirus bovino neurotrópico, ¿una enfermedad subdiagnosticada en Sudamérica?.[Neurotropic bovine astrovirus-associated encephalitis$bAn underdiagnosed disease in South America?].$h[electronic resource] 260 $c2022 500 $aArticle history: Recibido el 15 de julio de 2020/ Aaceptado el 24 de enero de 2021./Disponible en Internet el 18 junio 2021. Autor para correspondencia: Correo electrónico: fgiannitti@inia.org.uy (F. Giannitti). Este trabajo fue financiado por el proyecto PL 27 N-23398 y la beca de posgrado 1070-2018 del INIA, Uruguay. 520 $aResumen: Describimos un caso de encefalitis asociada a infección por astrovirus bovino neurotrópico en una vaca lechera, raza Jersey, del departamento de San José, Uruguay. Este representa el segundo caso reportado de esta condición en el hemisferio sur. La vaca, única afectada de un rodeo de 70 bovinos, manifestó signos clínicos neurológicos con curso de 2 días, luego de los que murió espontáneamente. El examen histopatológico reveló meningoencefalitis linfocítica, histiocítica y plasmacítica, con necrosis neuronal, sin cuerpos de inclusión. No se detectaron en el cerebro otros agentes infecciosos, incluyendo el virus de la rabia (Lyssavirus), alfaherpesvirus bovino-1 y alfaherpesvirus bovino-5 (Varicellovirus), virus de la diarrea viral bovina (Pestivirus), virus del Nilo Occidental (Flavivirus), Listeria monocytogenes, Histophilus somni y otras bacterias. Dado que el descubrimiento de astrovirus neurotrópicos en varias especies de mamíferos, incluidos humanos, es reciente, proponemos que los casos de encefalitis por astrovirus pudieron haber pasado inadvertidos en Sudamérica. Discutimos brevemente el diagnóstico patológico diferencial de encefalitis infecciosas en bovinos. bstract We describe a case of neurotropic bovine astrovirus-associated encephalitis in a Jersey dairy cow from the department of San José, Uruguay. This represents the second case of this condition reported in the Southern Hemisphere. The cow was the only one affected in a herd of 70 cows, showing neurological signs with a 2-day clinical course, before dying spontaneously. Histopathological examination revealed lymphocytic, histiocytic, and plasmacytic meningoencephalitis with neuronal necrosis, without detectable inclusion bodies. Other infectious agents, including Rabies virus (Lyssavirus), Bovine alphaherpesvirus-1 and Bovine alphaherpesvirus-5 (Varicellovirus), Bovine viral diarrhea virus (Pestivirus), West Nile virus (Flavivirus), Listeria monocytogenes, Histophilus somni and other bacteria, were not detected in the brain. We propose that given the recent discovery of neurotropic astroviruses in various mammalian species, including humans, cases of astrovirus encephalitis may have gone undetected in South America.We briefly discuss the differential pathologic diagnosis of infectious bovine encephalitis. Abstract We describe a case of neurotropic bovine astrovirus-associated encephalitis in a Jersey dairy cow from the department of San José, Uruguay. This represents the second case of this condition reported in the Southern Hemisphere. The cow was the only one affected in a herd of 70 cows, showing neurological signs with a 2-day clinical course, before dying spontaneously. Histopathological examination revealed lymphocytic, histiocytic, and plasmacytic meningoencephalitis with neuronal necrosis, without detectable inclusion bodies. Other infectious agents,including Rabies virus (Lyssavirus), Bovine alphaherpesvirus-1 and Bovine alphaherpesvirus-5 (Varicellovirus), Bovine viral diarrhea virus (Pestivirus), West Nile virus (Flavivirus), Listeria monocytogenes, Histophilus somni and other bacteria, were not detected in the brain. We propose that given the recent discovery of neurotropic astroviruses in various mammalian species,including humans, cases of astrovirus encephalitis may have gone undetected in South America. We briefly discuss the differential pathologic diagnosis of infectious bovine encephalitis. © 2021 Asociacion´ Argentina de Microbiolog´?a 650 $aURUGUAY 650 $aVACAS LECHERAS 653 $aAstrovirus bovino 653 $aBOVINE ASTROVIRUS 653 $aDAIRY COWS 653 $aEnfermedad neurológica 653 $aNeurological disease 653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL 653 $aPLATAFORMA DE SALUD ANIMAL 700 1 $aCASTELLS, M. 700 1 $aMAYA, L. 700 1 $aFRAGA, M. 700 1 $aUZAL, F.A 700 1 $aCOLINA, R. 700 1 $aGIANNITTI, F. 773 $tRevista Argentina de Microbiologia, Volume 54, Pages 100-105, 2022. OPEN ACCESS. Doi: https://doi.org/10.1016/j.ram.2021.01.00
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INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
11/08/2016 |
Actualizado : |
12/03/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
ROCHA, C.M.L.; VELLICCE, G.R.; GARCÍA, M.G.; PARDO, E.M.; RACEDO, J.; PERERA, M.F.; DE LUCÍA, A.; BONNECARRERE, M.; GERMAN, S.; MARCELINO, F.; LEDESMA, F.; REZNIKOV, S.; PLOPER, D.L.; WELIN, B.; CASTAGNARO, A.P. |
Afiliación : |
CARLA MARÍA LOURDES ROCHA, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; GABRIEL RICARDO VELLICCE, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; MARÍA GABRIELA GARCÍA, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; ESTEBAN MARIANO PARDO, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; JOSEFINA RACEDO, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; MARÍA FRANCISCA PERERA, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; ADRIÁN DE LUCÍA, INTA (Instituto Nacional de Tecnología Agropecuaria) - Cerro Azul; MARIA VICTORIA BONNECARRERE MARTINEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SILVIA ELISA GERMAN FAEDO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FRANCISMAR MARCELINO, EMBRAPA Soja; FRANCISCO LEDESMA, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; SEBASTIÁN REZNIKOV, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; LEONARDO DANIEL PLOPER, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; BJORN WELIN, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; ATILIO PEDRO CASTAGNARO, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino. |
Título : |
Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi. |
Fecha de publicación : |
2015 |
Fuente / Imprenta : |
Electronic Journal of Biotechnology, 2015, v. 18, no. 6, p. 439-444. OPEN ACCESS. |
DOI : |
10.1016/j.ejbt.2015.06.007 |
Idioma : |
Inglés |
Notas : |
Article history: Received 24 April 2015. Accepted 23 September 2015. Available online 28 October 2015. |
Contenido : |
ABSTRACT.
Background: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results: We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919
markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled. Conclusions: The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an important and potent molecular tool for the study of genetic diversity and could be useful to carry out wider genetic diversity studies.
© 2015 Pontificia Universidad Católica de Valparaíso. Production and hosting by Elsevier B.V. All rights reserve MenosABSTRACT.
Background: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results: We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919
markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled. Conclusions: The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an impor... Presentar Todo |
Palabras claves : |
ASIAN SOYBEAN RUST; GENETIC VARIATION; GLYCINE MAX; MOLECULAR MARKERS. |
Thesagro : |
MARCADORES MOLECULARES; SOJA; VARIACION GENETICA. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/5850/1/Rocha-C.M.-2015.-Electr.Jr.Biotech.-v18-p.439-444.pdf
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Marc : |
LEADER 02957naa a2200397 a 4500 001 1055241 005 2019-03-12 008 2015 bl uuuu u00u1 u #d 024 7 $a10.1016/j.ejbt.2015.06.007$2DOI 100 1 $aROCHA, C.M.L. 245 $aUse of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi.$h[electronic resource] 260 $c2015 500 $aArticle history: Received 24 April 2015. Accepted 23 September 2015. Available online 28 October 2015. 520 $aABSTRACT. Background: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results: We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919 markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled. Conclusions: The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an important and potent molecular tool for the study of genetic diversity and could be useful to carry out wider genetic diversity studies. © 2015 Pontificia Universidad Católica de Valparaíso. Production and hosting by Elsevier B.V. All rights reserve 650 $aMARCADORES MOLECULARES 650 $aSOJA 650 $aVARIACION GENETICA 653 $aASIAN SOYBEAN RUST 653 $aGENETIC VARIATION 653 $aGLYCINE MAX 653 $aMOLECULAR MARKERS 700 1 $aVELLICCE, G.R. 700 1 $aGARCÍA, M.G. 700 1 $aPARDO, E.M. 700 1 $aRACEDO, J. 700 1 $aPERERA, M.F. 700 1 $aDE LUCÍA, A. 700 1 $aBONNECARRERE, M. 700 1 $aGERMAN, S. 700 1 $aMARCELINO, F. 700 1 $aLEDESMA, F. 700 1 $aREZNIKOV, S. 700 1 $aPLOPER, D.L. 700 1 $aWELIN, B. 700 1 $aCASTAGNARO, A.P. 773 $tElectronic Journal of Biotechnology, 2015$gv. 18, no. 6, p. 439-444. OPEN ACCESS.
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